Does it have any disadvantages because of which it was not included in the post? The dye is red at pH 5 and blue at pH 3. India ink is good but can look grainy. Nigrosin gives a more even background and spreads little easier. One trick I used when I was doing this stain with Klebsiella was to grow the organism in a milk based media.
Word of caution: there is no heat fixing so need to be carful handling the slide since the organism has not been killed. Our Nigrosin does have formalin in it to act as a fixative. Then I cannot see the bacteria under a microscope. How do I avoid this? I believe that the crystal violet is the culprit, but I am not sure. This site uses Akismet to reduce spam.
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She raised a brow at him before smiling. He blinked a couple of times before letting out a rough chuckle. Jake let out a breath and followed her to the bar where she was already holding up two fingers at the bartender.
The creature starts off as a small statue and he can shrink to tiny size or grow to the size of a horse. June The pen rests are currently out of stock. The Last Mile. Use dark ink and then dilute it to indicate the lighter areas of your subject. I would like to reread it. It was paperback and though thin was oversized.
He sidled up to her side and noticed that Owen and Graham had moved a little farther down the bar, giving him space with this new development. He loved his brothers sometimes. Kind of hard to sound casual with a hard-on from hell pushing against his zipper. She smiled and held out a tequila shot. He took it and tapped glasses. Neither of them bothered with salt or lime, instead, letting it hit the back of their throats as they shot it back.
She smiled again. You were totally checking me out the entire time I was dancing, and you liked what you saw. Jake ran a hand over his day-old beard. Jake studied her face and forced himself not to reach out and touch. He had enough issues going on at home right now without adding women trouble to the mix. She shook her head. He sucks at oral. Now if this guy was as good as he thought he was in the sack, I might have. She held up her hands. Two men going at it is hot as hell.
I love men, and I love men that love men. Tonight…tonight was going to mean something. He could feel it.
He was going to taste every inch of her like he wanted to, and fuck her until they were both breathless. Probably more than two or three times for that matter.
She licked up his shoulder and neck then bit down gently where the two met. He groaned, rocking into her so his dick pressed against the heat of her pussy. For now. His brothers had left before he had, meaning he was all alone now with Maya in his home and in his arms. Maya pushed her breasts against his chest. Because I already told you, being shitty at oral is a deal breaker for me. He growled and pushed her harder into the door, using one hand to pin her wrists over her head and the other to hold her ass.
As for oral? How does that sound? She rocked herself on him, her legs wrapped around his waist. He took a moment to regain his control since he was about two seconds from blowing his load in his pants like a damn teenager. Once he could breathe again, he crushed his mouth to hers, needing her taste. Her tongue slid against his and she pulled back, biting his lip.
He growled, kissing her again, using his hips to hold her in place so he could use his free hand to cup her breast. I wonder what color your nipples are. They both wanted this, and hell, if he were honest with himself, he needed this. He slowly undid her top from the back and let it fall to the floor. Her nipples were a dusty pink, slightly large on her ample breasts, and fucking hard as hell. Jake bent down, taking a nipple into his mouth and using his free hand to cup her other breast.
Maya put her hand on the back of his head and pressed him closer.
He did as she asked and moved his hand down her belly and over her skirt before sliding it back up again, this time under the fabric. When his fingers brushed her damp panties, they both let out a groan. He moved to her other breast and pushed her panties aside with his fingers, needing to feel her. He slid his finger over her clit slightly before spearing her with two fingers. He took that as a challenge and fucked her hard with his fingers. She threw her head back, and that made her breasts push toward him.
Grinning at his luck, he took her nipple between his teeth and bit down. She came around his fingers, shouting his name and rocking her hips until she shook. He slid his hand up her bare back and to her head so he could bring her in for a kiss. When he slid his hand out from beneath her skirt and licked his fingers, she smiled. He let her fall to her knees in front of him and had to swallow hard as she undid the button of his jeans. He slid his hand through her hair, and she smiled up at him before licking up his shaft. That damn tongue ring would be his undoing.
He shuddered and had to hold himself back from coming right then and there. She laughed before slowly sucking on his balls. He swallowed hard and tried to keep his eyes open.
When she licked the tip of his cock, he groaned. Now let me get to work. She worked him, her tongue ring dancing along his dick as she sucked him.
He rocked his hips, unable to hold back, but when she hummed against him, he knew she liked it, too. She fondled his balls with one hand and reached around to his ass to run her fingers along his crack. Tell me where you want it. She looked up at him, big eyes and all hotness. The first spurt hit her tongue, and he roared her name, his body shaking as he came. Jake was about to bend down and pick her up so he could kiss her once more before going down on her when someone slammed their fist on the door. Murphy passed out. You need to get dressed, say goodbye to the chick, and get in the fucking car.
Maya looked at him questioningly even as she helped him zip up his pants, tucking his still hard cock, wet from her mouth, away. He saw the questions in her gaze and knew he had to at least tell her something. She kissed him softly and patted his chest. Go to your brother.